- Product name: Pleckstrin (PLEK), ELISA kit
- Full Product Name: Human Pleckstrin ELISA Kit (PLEK)
- Product gene name: PLEK ELISA Kit
- OMIM: X07743 mRNA
- 3D structure: ModBase 3D Structure for P08567
- Species reactivity: Human
Specificity
This assay has high sensitivity and excellent specificity for the detection of human PDGFD. No significant cross-reactivity or interference was observed between human PDGFD and its analogs.
Samples: Serum, plasma, and other biological fluids
Test type: Quantitative sandwich
Detection range: 0.31 ng / ml-20 ng / ml
Sensitivity: 0.156 ng/ml (mean of 6 independent tests).
Intra-assay precision
Intra-assay precision (within-assay precision) Three samples of known concentration were run twenty times on a plate to assess intra-assay precision. Intra-assay: CV <8%
Interassay precision
Interassay Precision (Between Assay Precision) Three samples of known concentration were analyzed in forty separate assays to assess interassay precision. CV (%) = SD / mean X100. Interassay: CV <12%
Preparation and storage
Store at 2-8 degrees C.
Product note
Information selected from the online data sheet is extracted from bioinformatics databases, occasionally resulting in ambiguous or irrelevant product information. It is the customer’s responsibility to review, verify and evaluate the information to make sure it matches their requirements before purchasing the kit. The essays in our ELISA kit are dynamic research tools and can sometimes be updated and improved.
If the format of this assay is important to you, please request the current manual or contact our technical support team with a pre-sale consultation before placing an order. We will confirm the current details of the trial. We cannot guarantee that the sample manual posted online is the most current manual, it is intended to serve as an example only. See the instructions for use provided with the test kit for precise details.
Other notes
Occasionally, small volumes of the PLEK ELISA kit vials can get caught in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Some products may need to be shipped with dry ice and an additional fee may apply for dry ice.
Search terms for PLEK purchases
MBS281194 is a ready-to-use microwell plate ELISA (Enzyme-Linked Immunosorbent Assay) kit for testing the presence of Pleckstrin ELISA Kit (PLEK) target analytes in biological samples. The concentration gradients of the kit standards or positive controls provide a theoretical detection range of the kit in biological research samples containing PLEK.
The analytical biochemical ELISA technique of the MBS281194 kit is based on PLEK antibody-antigen interactions PLEK (immunosorbent) and an HRP colorimetric detection system to detect PLEK antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant PLEK. Appropriate sample types may include secretions and undiluted body fluids and/or tissue homogenates. Quality control trials evaluating reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
Related Product Information for PLEK ELISA kit
Intended Uses: For the quantitative detection of the concentration of human platelet-derived growth factor D (PDGFD) in serum, plasma, and other biological fluids.
Assay principle: This assay employs a two-site sandwich ELISA to quantify PDGFD in human serum, plasma. A microplate has been pre-coated with an antibody specific for PDGFD. Standards and samples are pipetted into the wells and any PDGFD present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFD is added to the wells.
After washing, streptavidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and the color develops in proportion to the amount of PDGFD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Precautions
All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic, or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order when we reasonably believe that the intended use will be outside of our acceptable guidelines.
Disclaimer
While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be responsible for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
