The Anigen Rapid Rabies Ag Test Kit is a chromatographic immunoassay for the qualitative detection of rabies virus antigen in canines, bovines, raccoon dog saliva secretions, and brain homogenates. The Anigen Ag Rapid Rabies Test Kit has a letter of “T” and “C” as proof line and control line on the surface of the device. Both the test line and the control line in the results window are not visible before applying the samples.
The control line is used for procedural control. The control line must always appear if the test procedure is successful and the test control line reagents are working. A purple test line will be visible on the result window if there is enough rabies virus antigen in the sample. Specially selected rabies virus antibodies are used in the test band as capture and detection materials. These enable the Anigen Rapid Rabies Ag Test Kit to identify rabies virus antigen in canines, cattle, and raccoons saliva secretion from the dog, and the brain is homogenized with a high degree of precision.
Materials provided (10 tests/kit)
- Ten (10) Anigen Ag Rabies Rapid Test Kits
- Ten (10) sample tubes containing Assay Diluent Buffer
- Ten (10) sample collection swabs
- Ten (10) disposable droppers
- One (1) instruction for use
- For veterinary diagnostic use only.
- For best results, strict adherence to instructions is required.
- All samples must be handled as potentially infectious.
- Do not open or remove the test kit from its individually sealed bags until immediately before use.
- Do not use the test kit if the bag is damaged or the seal is broken.
- Do not reuse the test kit.
- All reagents must be at room temperature before running the assay.
- Do not use reagents after the indicated expiration date marked on the label.
- The components in this kit have undergone quality control testing as standard batch units.
Storage and stability
The kit can be stored at room temperature (2 ~ 30) or refrigerated. ℃ The test kit is stable until the expiration date is marked on the package label. MAKE DO NOT FREEZE. Do not store the test kit in direct sunlight.
Sample collection and preparation
- Saliva samples and brain homogenates should be used for this proof.
- Samples should be analyzed immediately as soon as samples are collected. of canine, bovine, and raccoon dogs.
- Collect the saliva samples and brain homogenates using the swab.
- Insert the swab into the sample tube containing 1 ml of Assay Diluent.
- Mix the swab samples with Assay Diluent to extract well.
- Remove the test device from the foil pouch and place it on a flat, dry surface.
- Using the disposable dropper provided, take the extracted samples and mixed samples in the tube.
- Add four (4) drops to the sample hole with the disposable dropper.
- As the test starts to work, you will see the purple color move through the result window in the center of the test device. If the migration has not appeared after 1 minute, add one more drop of the Mixed Assay Diluent to the sample well.
- Interpret the test results at 5 ~ 10 minutes. Don’t decide after 10 minutes.
Interpretation of the test
A colored band will appear in the left section of the results window to show that the test is working correctly. This band is the control band. The correct section in the results window indicates the test results. If another color band appears in the right section of the results window. This band is the test band.
- Negative result: The presence of a single band within the results window indicates a negative result.
- Positive result: The presence of two color bands (“T” and “C”) within the results window, no matter which band appears first, indicates a positive result.
- Invalid result: If the purple band is not visible within the results window after when performing the test, the result is considered invalid. Addresses can it has not been followed correctly or the test may have deteriorated. It is recommended that the sample be retested.
Limitations of the test
Although the Anigen Rapid Rabies Ag Test Kit is very accurate in detecting Rabies virus antigen, a low incidence of false results may occur. Other clinically available tests are required if questionable results are obtained what with all diagnostic tests, a definitive clinical diagnosis should not be based on the results of a single test, but should only be performed by the vet, after All, clinical and laboratory findings have been evaluated. The detection limit of this kit is about 102.0LD50 / 0.03ml in mice.