- Product name: IgM ELISA Kit for SARS-CoV-2
- Detection method: Colorimetric
- Example type: Serum, EDTA plasma, Cit plasma, lithium heparin plasma
- Test type: Semi-quantitative
- Trial duration: Standard multi-step test
The IgM ELISA Kit for SARS-CoV-2 (ab275299) is an enzyme-linked immunosorbent assay (ELISA) for the semi-quantitative detection of IgM antibodies against SARS-CoV-2 in human serum or plasma collected in potassium EDTA, citrate sodium, or lithium. heparin.
This kit is designed to help identify people with an adaptive immune response to SARS-CoV-2, indicating previous or recent infection. Understanding the timing, duration, and efficacy of humoral immune responses in people previously infected with SARS-CoV-2 will be important for epidemiological and vaccine research. At this time, it is unknown how long antibodies persist after infection and whether the presence of antibodies confers protective immunity.
The results correspond to the detection of antibodies against SARS-CoV-2. IgM antibodies to SARS-CoV-2 are generally detected in the blood several days after initial infection, although the length of time that antibodies are present after infection is not well characterized. Individuals can have a detectable virus for several weeks after seroconversion.
The sensitivity of the SARS-CoV-2 IgM ELISA shortly after infection is unknown. Negative results do not exclude acute SARS-CoV-2 infection. False-positive results for the SARS-CoV-2 IgM ELISA may occur due to cross-reactivity of pre-existing antibodies to SARS-CoV-1 or other possible causes.
This kit provides an indirect ELISA, in which a recombinant receptor-binding domain (RBD) of the SARS-CoV 2 Spike1 protein is coated in the wells of the microtiter plate. Antibodies against SARS-CoV-2 RBD when present in the test sample specifically bind to the RBD protein. After sample binding, unbound proteins and molecules are washed and an HRP-conjugated detection antibody is added to the wells to bind the captured anti-SARS-CoV2 IgM antibodies. Then the chromogenic substrate TMB (3.3 ‘, 5.5’ tetramethylbenzidine) is added.
This reaction produces a blue product, which turns yellow when the reaction is terminated by the addition of dilute sulfuric acid. The absorbance of the yellow product at 450 nm, corrected for plate imperfections by subtracting the absorbance at 570 nm, is proportional to the amount of RBD-specific anti-SARS-CoV-2 IgM present in the sample.
After the positive control and negative control, values are determined to be valid and acceptable by comparing them to the calibrator value, the sample values are compared to the calibrator to generate a relationship. Proportions above a limit indicate positive samples and values below a limit indicate negative samples.
Precoated Microplate (12 x 8-well strips)